Parameter unit is convertible for user''s convenience.Vacuum pump usage time is recorded for timely maintenance. Data management by USB (Touch optional) Drying time record is stored.
#Lyophilizer freeze dryer Pc#
Operation status can be monitored by PC through RS/232(485) communication port.Graphic display of condenser temperature and vacuum level is provided.
#Lyophilizer freeze dryer password#
Allow approximately 15 minutes to pass, then return to ensure the pressure inside has reached below 100 mTorr.įill out the sign-in sheet. As the pressure decreases, check to see that all valves on top are closed (you can use a finger to check for suction or you can listen for a hissing noise. IMPORTANT: Watch the vacuum pressure on the freeze dryer until the pressure reading is below 100 Torr, this should be a fairly rapid process. Turn on vacuum by pressing the 'vacuum' button. The vacuum pump does not want liquid water moving through it, so please be patient and wait. It is critical that you wait to start the vacuum pump until the condenser is below -40 ☌. Your sample material could creep up or even explode out the top of a vial, so take the time to secure your material inside the vial.
You may need to use dry ice or liquid nitrogen to get your samples to freeze if they are rich in solutes.Ĭonsider placing paper towel or kim wipe on top of an open sample container secured with a rubber band. If your samples are particularly wet, freeze them before putting them in the freeze dryer and keep them frozen while waiting for condenser to reach -40 ☌. If you are using the tree, place the bottom acrylic plate specific for the tree on top of the condenser basin then place the tree with white o-ring on top of the plate. If you are going to use the drum, make sure the metal shelves are spaced appropriately for your samples. Look in the cabinet shown in the photo to find supplies. While you are waiting for the temperature to drop below -40 ☌, decide if you are using the drum or the tree. With a dry condenser basin and the drain hose removed, make sure the unit has power (power switch on back) and turn on the condenser by pressing the 'condenser' button. If the condenser has liquid water in it, see clean up section below.
#Lyophilizer freeze dryer free#
The condenser should be dry and free of water (liquid or solid) before starting a new session. Write name, date, contents, expected completion date on sign-in sheet.Įxhaustive Protocol Prepare the Freeze Dryer.When the condenser is below -40 ☌, turn on vacuum.If using shelves, put large acrylic cover (drum) over samples.Load frozen samples onto shelves or into glass vessels.Choose from the drum with shelves or tree with vessels.If you are venting the freeze dryer, do so slowly! Do not rapidly vent the drum. Given the freeze dryer might be under vacuum or the condenser might be very cold, caution should be taken when working in and around this unit. Our freeze dryer is a Virtis BT4KXL-75 with a Leybold Trivac D2.5E vacuum pump. Samples are frozen, not by actively freezing as in a freezer, but by energy loss through sublimation. Through sublimation and low vapor pressure, water is extracted from samples and frozen on the condenser. The freeze dryer, or lyophilizer, is a quick and efficient way to dry samples.
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